Genome of Sindbis Virus
نویسندگان
چکیده
منابع مشابه
Genome of Sindbis virus.
(32)P-labeled ribonucleic acid (RNA) from purified Sindbis virus was examined for the presence of hidden breaks. Viral RNA was treated with acid at pH 2.9 or with formaldehyde and was analyzed on sucrose gradients or by polyacrylamide gel electrophoresis. The sedimentation pattern and mobility on polyacrylamide gels of the 42S RNA was unaffected by heating and quick cooling and was not altered ...
متن کاملSindbis virus with a tricomponent genome.
We established a system for propagation of Sindbis virus (SIN)-based replicons in tissue culture in the form of a tricomponent genome virus. Three RNA fragments containing complementing genetic information required for virus replication are packaged into separate viral particles, and each cell produces at least 1,000 packaged replicons and the number of packaged helpers sufficient to perform th...
متن کاملPhosphorylated proteins of Sindbis virus.
The capsid and two membrane proteins of Sindbis virus, grown in chicken cells, contain 0.03 to 0.1 mol of phosphate per mol of protein.
متن کاملAgglutination of Sindbis virus and of cells infected with Sindbis virus by plant lectins.
We have examined the agglutination of Sindbis virus and of chick and hamster cells infected with Sindbis virus by two of the plant lectins, concanavalin A and Ricinus communis agglutinin. Both lectins agglutinate the virus by binding to the polysaccharide chains of the envelope glycoproteins. Both chick and hamster cells exhibit increased agglutination by the lectins after infection by Sindbis ...
متن کاملAttenuation of Sindbis virus neurovirulence by using defined mutations in nontranslated regions of the genome RNA.
We examined a panel of Sindbis virus mutants containing defined mutations in the 5' nontranslated region of the genome RNA, in the 3' nontranslated region, or in both for their growth in cultured cells and virulence in newborn mice. In cultured cells, these viruses all had defects in RNA synthesis and displayed a wide range of growth rates. The growth properties of the mutants were often very d...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1972
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.9.1.102-109.1972